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KMID : 1094719990040030219
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 3 p.219 ~ p.223
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Inhibition of growth and adhesion ofHelicobacter pylori using egg yolk antibodies
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Koo Jae-Kyoung
Kim Chang-Hwan
Choe Tae-Boo
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Abstract
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Helicobacter pylori is known as a key pathogen for chronic gastric and duodenal ulcers. Egg yolk antibody, IgY produced from chicken immunized withH. pylori antigen was tested for the inhibition of growth and adhesion ofH. pylori to gastric epithelial cell, AGS. The colony forming ofH. pylori was repressed by 30% using 1 mg/mL of IgY while that ofE. coli was only 7% with the same amount of IgY, which showed the growth inhibition ofH. pylori was mainly due to the specific interaction between IgY andH. pylori. The inhibition ofH. pylori adhesion to AGS was as high as 90% using 0.5 mg/mL of antibody only. More than 80% ofH. pylori attached to AGS could be detached treating with the same amount of IgY for one and a half hr. However, this effect was severely dependant on theH. pylori strains tested. The strain used for immunization of chicken was very sensitive to the antibody treatment but changing the test strain generally showed a variation in adhesion inhibition between 15 and 80%. Further studies are necessary to employ the egg yolk antibodies for the treatment ofH. pylori in vivo.
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KEYWORD
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Helicobacter pylori, growth inhibition, binding inhibition, egg yolk antibody
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